Nicotinamide metabolism in mammals

Nicotinamide metabolism in mammals.

Lysine metabolism in mammals.

The enzymes involved in the initial degradative steps of lysine metabolism, lysine-2-oxoglutarate reductase and saccharopine dehydrogenase, were studied and their activities in different mammals compared. Values obtained in human, rat, pig, dog, cat, ox and sheep liver indicated that in vitro, appreciable degradation of lysine to saccharopine (4-6nmol/min per mg of protein) occurred. The specif...

Nicotinamide metabolism regulates glioblastoma stem cell maintenance.

Metabolic dysregulation promotes cancer growth through not only energy production, but also epigenetic reprogramming. Here, we report that a critical node in methyl donor metabolism, nicotinamide N-methyltransferase (NNMT), ranked among the most consistently overexpressed metabolism genes in glioblastoma relative to normal brain. NNMT was preferentially expressed by mesenchymal glioblastoma ste...

Enzymes of Nuclear Nicotinamide-Adenine Dinucleotide Metabolism

Changing the concentrations of NAD+ and histones did not alter significantly the observed inhibition. However, the inhibition was relieved by increasing amounts of DNA (Fig. 36). The effect of Actinomycin D was further studied by changing the order of addition of the various compounds (Fig. 3c). These results showed that when Actinomycin D was incubated with the polymerase before the addition o...

Nicotinamide riboside kinases display redundancy in mediating nicotinamide mononucleotide and nicotinamide riboside metabolism in skeletal muscle cells

OBJECTIVE Augmenting nicotinamide adenine dinucleotide (NAD+) availability may protect skeletal muscle from age-related metabolic decline. Dietary supplementation of NAD+ precursors nicotinamide mononucleotide (NMN) and nicotinamide riboside (NR) appear efficacious in elevating muscle NAD+. Here we sought to identify the pathways skeletal muscle cells utilize to synthesize NAD+ from NMN and NR ...